Journal article
Laboratory evolution of synthetic electron transport system variants reveals a larger metabolic respiratory system and its plasticity
The bacterial respiratory electron transport system (ETS) is branched to allow condition-specific modulation of energy metabolism. There is a detailed understanding of the structural and biochemical features of respiratory enzymes; however, a holistic examination of the system and its plasticity is lacking.
Here we generate four strains of Escherichia coli harboring unbranched ETS that pump 1, 2, 3, or 4 proton(s) per electron and characterized them using a combination of synergistic methods (adaptive laboratory evolution, multi-omic analyses, and computation of proteome allocation). We report that: (a) all four ETS variants evolve to a similar optimized growth rate, and (b) the laboratory evolutions generate specific rewiring of major energy-generating pathways, coupled to the ETS, to optimize ATP production capability.
We thus define an Aero-Type System (ATS), which is a generalization of the aerobic bioenergetics and is a metabolic systems biology description of respiration and its inherent plasticity.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2022 |
Pages: | 3682 |
ISSN: | 20411723 |
Types: | Journal article |
DOI: | 10.1038/s41467-022-30877-5 |
ORCIDs: | 0000-0003-2089-1288 , 0000-0002-4122-6589 , Feist, Adam M. and Palsson, Bernhard O. |