Journal article
Improved diagnosis for nine viral diseases considered as notifiable by the World Organization for Animal Health
Complutense University1
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark2
Division of Virology, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
National Veterinary Institute5
Pirbright Institute6
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia7
Queen's University Belfast8
Research Center Borstel9
Nine viral diseases included in the World Organization for Animal Health list of notifiable diseases (former list A) were chosen for their contagiousness and high capacity of spreading to improve their diagnosis using new and emerging technologies. All the selected diseases--foot-and-mouth disease, swine vesicular disease, vesicular stomatitis, classical swine fever, African swine fever, bluetongue, African horse sickness, Newcastle disease and highly pathogenic avian influenza--are considered as transboundary diseases, which detection causes the prohibition of livestock exportation, and, thus, it leads to high economical losses.
The applied diagnostic techniques can fall into two categories: (i) nucleic-acid detection, including padlock probes, real-time PCR with TaqMan, minor groove binding probes and fluorescence energy transfer reaction probes, isothermal amplification like the Cleavase/Invader assay or the loop-mediated amplification technology and the development of rapid kits for 'mobile' PCR and (ii) antigen-antibody detection systems like simplified and more sensitive ELISA tests.
Besides, internal controls have been improved for nucleic acid-detecting methods by using an RNA plant virus--Cowpea Mosaic Virus--to ensure the stability of the RNA used as a positive control in diagnostic real-time RT-PCR assays. The development of these diagnosis techniques has required the joint efforts of a European consortium in which nine diagnostic laboratories and an SME who have collaborated since 2004 within the European Union-funded Lab-on-site project.
The results obtained are shown in this paper.
Language: | English |
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Year: | 2008 |
Pages: | 215-225 |
ISSN: | 18651682 and 18651674 |
Types: | Journal article |
DOI: | 10.1111/j.1865-1682.2008.01037.x |
ORCIDs: | Rasmussen, Thomas Bruun |