Journal article
WCS120 protein family and proteins soluble upon boiling in cold-acclimated winter wheat
The amount of proteins soluble upon boiling (especially WCS120 proteins) and the ability to develop frost tolerance (FT) after cold acclimation was studied in two frost-tolerant winter wheat cultivars, Mironovskaya 808 and Bezostaya 1. Protein get Not analysis, mass spectrometry (MS) and image analysis of two-dimensional get electrophoresis (2-DE) gets were used to identify and/or quantify the differences in protein patterns before (non-acclimated, NA) and after 3 weeks of cold acclimation (CA) of the wheats, when FT increased from -4 degrees C (Lethal, temperature (LT50), for both cultivars) to -18.6 degrees C in Bezostaya 1 and -20.8 degrees C in Mironovskaya 808.
Only WCS120 protein was visible in NA leaves white all five WCS120 proteins were induced in the CA Leaves. Mironovskaya 808 had higher accumulation of three members of WCS120 proteins (WCS120, WCS66 and WCS40) than Bezostaya 1. MS analysis of total sample of proteins soluble upon boiling showed seven COR proteins in the CA samples and only three COR proteins in the NA samples of cultivar Mironovskaya 808 (MIR).
In conclusion, the Level of the accumulation of WCS120, WCS66 and WCS40 distinguished our two frost-tolerant winter wheat cultivars. Moreover, the differences of CA and NA samples of the MIR were shown by Liquid chromatography (LC)-tandem mass spectrometry (MS/MS). (c) 2006 Etsevier GmbH. All rights reserved.
| Language: | English |
|---|---|
| Year: | 2007 |
| Pages: | 1197-1207 |
| ISSN: | 16181328 and 01761617 |
| Types: | Journal article |
| DOI: | 10.1016/j.jplph.2006.06.011 |
2-DE - two-dimensional gel electrophoresis Acclimatization BEZ - cultivar Bezostaya 1 CA - cold-acclimated (cultivation at 2°C) COR - cold-regulated Cold Temperature DNA-Binding Proteins FT - frost tolerance Hot Temperature LC - liquid chromatography LEA - late embryonic abundant LT50 - lethal temperature MIR - cultivar Mironovskaya 808 MS - mass spectrometry MS/MS - tandem mass spectrometry Multigene Family NA - non-acclimated (cultivation at 17°C) Plant Proteins Q - quadrupoles QM - queries matched SDS-PAGE - sodium dodecyl sulfate polyacrylamide gel electrophoresis Solubility TOF - time-of-flight Triticum Wcs120 protein, Triticum aestivum m/z - mass-to-charge ratio
