Journal article
Production of naphthalene-cis-glycol by Pseudomonas putida in the presence of organic solvents
Advanced Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, London, UK
The effect of water-immiscible organic solvents on the conversion of naphthalene to naphthalene-1,2-dihydro-1,2-diol by Pseudomonas putida has been examined. The bacteria were stable in the presence of dodecane, giving a maximum reaction rate (about 1 g g dcw−1 h−1) with naphthalene concentrations greater than 40 g l−1 dodecane.
For solvents with log P values less than 3.5, no activity was observed. Immobilization in calcium alginate beads improved the stability of bacteria in the presence of solvents with log P values of 3.5–4. For solvents with log P values greater than 4, there was no activity loss during biotransformations lasting 5 h with either free or immobilized bacteria.
The results are compared with those obtained previously for steroid Δ1-dehydrogenation by Arthrobacter simplex in aqueous/organic reaction mixtures, and some general conclusions are drawn.
| Language: | English |
|---|---|
| Year: | 1991 |
| Pages: | 725-730 |
| ISSN: | 18790909 and 01410229 |
| Types: | Journal article |
| DOI: | 10.1016/0141-0229(92)90112-2 |
