Journal article
Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic
Department of Micro- and Nanotechnology, Technical University of Denmark1
Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark2
Center for Nanomedicine and Theranostics, Centers, Technical University of Denmark3
Department of Chemistry, Technical University of Denmark4
Physical and Biophysical Chemistry, Department of Chemistry, Technical University of Denmark5
Cationic membrane-active peptides have been studied for years in the hope of developing them into novel types of therapeutics. In this article, we investigate an effect that might have significant experimental implications for investigators who wish to study these peptides, namely, that the peptides adsorb to solid surfaces of glass and plastic.
Specifically, we use analytical HPLC to systematically quantify the adsorption of the three cationic membraneactive peptides mastoparan X, melittin, and magainin 2 to the walls of commonly used glass and plastic sample containers. Our results show that, at typical experimental peptide concentrations, 90% or more of the peptides might be lost from solution due to rapid adsorption to the walls of the sample containers.
Thus, our results emphasize that investigators should always keep these adsorption effects in mind when designing and interpreting experiments on cationic membrane-active peptides. We conclude the article by discussing different strategies for reducing the experimental impact of these adsorption effects.
Language: | English |
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Publisher: | Public Library of Science |
Year: | 2015 |
Pages: | e0122419 |
ISSN: | 19326203 |
Types: | Journal article |
DOI: | 10.1371/journal.pone.0122419 |
ORCIDs: | Kristensen, Kasper , Henriksen, Jonas Rosager and Andresen, Thomas Lars |