Dataset
Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes
Year: | 2017 |
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Types: | Dataset |
DOI: | 10.1371/journal.pone.0177591 |
Arabidopsis thaliana Arabidopsis thaliana cell wall glycosyltransferases Biochemistry Biological Sciences not elsewhere classified Biophysics Biotechnology Chemical Sciences not elsewhere classified DNA Genetics Plant Biology Reversibly Glycosylated Polypeptide 1 arabinopyranose mutase autoglycosylation activities biofuel crops cell wall glycosyltransferase production efforts cell wall glycosyltransferases co-expression partners enzymes Molecular characterization expression conditions expression organism expression screening heterologous expression cultures high-throughput expression screening lysis buffer optimization milligram amounts plant cell wall plant cell wall glycosyltransferases protein sequences protein solubility purification methods screening results silico selection target enrichment