Journal article
An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies
National Veterinary Institute, Technical University of Denmark1
Animal Health and Veterinary Laboratories Agency2
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail3
Section for Epidemiology, National Veterinary Institute, Technical University of Denmark4
BACKGROUND: Avian influenza virus (AIV) subtypes H5 and H7 attracts particular attention because of the risk of their potential pathogenicity in poultry. The haemagglutination inhibition (HI) test is widely used as subtype specific test for serological diagnostics despite the laborious nature of this method.
However, enzyme-linked immunosorbent assays (ELISAs) are being explored as an alternative test method.H5 and H7 specific monoclonal antibodies were experimentally raised and used in the development of inhibition ELISAs for detection of serological response specifically directed against AIV subtypes H5 and H7.
The ELISAs were evaluated with polyclonal chicken anti-AIV antibodies against AIV subtypes: H1N2, H5N2, H5N7, H7N1, H7N7, H9N9, H10N4 and H16N3. RESULTS: Both the H5 and H7 ELISA proved to have a high sensitivity and specificity and the ELISAs detected H5 and H7 antibodies earlier during experimental infection than the HI test did.
The reproducibility of the ELISA’s performed at different times was high with Pearson correlation coefficients of 0.96-0.98. CONCLUSIONS: The ELISAs are a potential alternative to the HI test for screening of large amounts of avian sera, although only experimental sera were tested in this study.
| Language: | English |
|---|---|
| Publisher: | BioMed Central |
| Year: | 2013 |
| Pages: | 84-84 |
| ISSN: | 17510147 and 0044605x |
| Types: | Journal article |
| DOI: | 10.1186/1751-0147-55-84 |
Animals Antibodies, Monoclonal Antibodies, Viral Avian influenza Chickens Enzyme-Linked Immunosorbent Assay Experimental sera H5 H7 Haemagglutination inhibition test Hemagglutinin Glycoproteins, Influenza Virus Influenza A virus Influenza in Birds Inhibition ELISA Monoclonal antibody Reproducibility of Results Sensitivity and Specificity Serology Specific Pathogen-Free Organisms hemagglutinin, avian influenza A virus
