Journal article
Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis
Department of Microbiology, Technical University of Denmark1
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark2
National Food Institute, Technical University of Denmark3
Communications and Management Secretariat, National Food Institute, Technical University of Denmark4
Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and Smal pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands.
By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.
| Language: | English |
|---|---|
| Year: | 2000 |
| Pages: | 677-680 |
| ISSN: | 14602091 and 03057453 |
| Types: | Journal article |
| DOI: | 10.1093/jac/45.5.677 |
| ORCIDs: | Aarestrup, Frank Møller and Wegener, Henrik Caspar |
Animals Anti-Bacterial Agents Bacterial Proteins Bacterial Typing Techniques CCCGGG-specific type II deoxyribonucleases Carbon-Oxygen Ligases Chickens Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific Electrophoresis, Gel, Pulsed-Field Enterococcus faecium Gram-Positive Bacterial Infections Humans Poultry Diseases Restriction Mapping Swine Swine Diseases VanA ligase, Bacteria Vancomycin Vancomycin Resistance
