Journal article
Diffusion studies of nanometer polymersomes across tissue engineered human oral mucosa
Biomaterials and Tissue Engineering Group, Department of Engineering Materials, Kroto Research Institute, North Campus, University of Sheffield, Broad Lane, Sheffield S37HQ, UK.1
To measure the diffusion of nanometer polymersomes through tissue engineered human oral mucosa. In vitro models of full thickness tissue engineered oral mucosa (TEOM) were used to assess the penetration properties of two chemically different polymersomes comprising two of block copolymers, PMPC-PDPA and PEO-PDPA.
These copolymers self-assemble into membrane-enclosed vesicular structures. Polymersomes were conjugated with fluorescent rhodamine in order to track polymersome diffusion. Imaging and quantification of the diffusion properties were assessed by confocal laser scanning microscopy (CLSM). TEOM is morphologically similar to natural oral mucosa.
Using CLSM, both formulations were detectable in the TEOM within 6 h and after 48 h both penetrated up to 80 microm into the TEOM. Diffusion of PMPC-PDPA polymersomes was widespread across the epithelium with intra-epithelial uptake, while PEO-PDPA polymersomes also diffused into the epithelium. CLSM was found to be an effective and versatile method for analysing the level of diffusion of polymersomes into TEOM.
The penetration and retention of PMPC-PDPA and PEO-PDPA polymersomes means they may have potential for intra-epithelial drug delivery and/or trans-epithelial delivery of therapeutic agents.
Language: | English |
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Publisher: | Springer US |
Year: | 2009 |
Pages: | 1718-1728 |
Journal subtitle: | An Official Journal of the American Association of Pharmaceutical Scientists |
ISSN: | 1573904x and 07248741 |
Types: | Journal article |
DOI: | 10.1007/s11095-009-9882-6 |
Biochemistry, general Biological Transport Biomedical Engineering Biomedicine Cells, Cultured Diffusion Drug Carriers Fibroblasts Humans Keratinocytes Medical Law Methacrylates Microscopy, Confocal Mouth Mucosa Pharmacology/Toxicology Pharmacy Phosphorylcholine Polyethylene Glycols Polymethacrylic Acids Tissue Engineering confocal laser scanning microscopy diffusion epithelium oral mucosa poly(2-(diisopropylamino)ethyl methacrylate) poly(2-methacryloyloxyethyl-phosphorylcholine) polymersome