Journal article
A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers
Department of Biotechnology and Biomedicine, Technical University of Denmark1
National Veterinary Institute, Technical University of Denmark2
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Bacterial Ecophysiology and Biotechnology, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Antimicrobial Agents and microbial ecology, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Bacteriology & Parasitology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark6
National Food Institute, Technical University of Denmark7
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark8
MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs.
On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus.
Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2018 |
Pages: | 12751 |
ISSN: | 20452322 |
Types: | Journal article |
DOI: | 10.1038/s41598-018-30689-y |
ORCIDs: | Strube, Mikael Lenz and Hansen, Julie Elvekjær |