Journal article
A high-quality catalog of the Drosophila melanogaster proteome
Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications.
This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics data provide crucial information to amend genome annotation and to confirm many predicted gene models.
We also present experimentally identified proteotypic peptides matching approximately 50% of D. melanogaster gene models. This library of proteotypic peptides should enable fast, targeted and quantitative proteomic studies to elucidate the systems biology of this model organism.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2007 |
Pages: | 576-83 |
Journal subtitle: | Science and Business of Biotechnology |
ISSN: | 15461696 and 10870156 |
Types: | Journal article |
DOI: | 10.1038/nbt1300 |