Journal article
Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis
Chalmers University of Technology1
Yeast Metabolic Engineering, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
University of Copenhagen4
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Goethe University Frankfurt6
Swedish University of Agricultural Sciences7
The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae.
The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose.
At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.
| Language: | English |
|---|---|
| Publisher: | Oxford University Press |
| Year: | 2019 |
| Pages: | 1-9 |
| ISSN: | 15746968 and 03781097 |
| Types: | Journal article |
| DOI: | 10.1093/femsle/fnz222 |
| ORCIDs: | Møller-Hansen, Iben , Darbani, Behrooz , Borodina, Irina , 0000-0001-5904-5885 and 0000-0001-6660-0509 |
Bioethanol Brettanomyces bruxellensis Glucose transport High-affinity Metabolism Xenopus laevis Yeast
Base Sequence Biological Transport Brettanomyces Carbohydrate Metabolism Cloning, Molecular Ethanol Fermentation Gene Expression Glucose Glucose Transport Proteins, Facilitative Multigene Family Oocytes Phylogeny Sequence Analysis, Protein bioethanol glucose transport high-affinity metabolism yeast
