Journal article
Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses
iLoop, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Synthetic Biology Tools for Yeast, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
University of California at San Diego5
ALE Technology & Software Development, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6
Network Reconstruction in Silico Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark7
Global Econometric Modeling, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark8
Big Data 2 Knowledge, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark9
We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution.
We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Language: | English |
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Publisher: | Public Library of Science |
Year: | 2019 |
Pages: | e2007050 |
ISSN: | 15457885 and 15449173 |
Types: | Journal article |
DOI: | 10.1371/journal.pbio.2007050 |
ORCIDs: | Luo, Hao , Hansen, Anne Sofie Lærke , Yang, Lei , Schneider, Konstantin , Kristensen, Mette , Christensen, Hanne Bjerre , Özdemir, Emre , Feist, Adam M , Jensen, Michael Krogh , Herrgård, Markus J and Palsson, Bernhard O |