Journal article
Lab-on-a-disk extraction of PBMC and metered plasma from whole blood: An advanced event-triggered valving strategy
Department of Health Technology, Technical University of Denmark1
Dublin City University2
Nanoprobes, Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark3
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark4
In this paper, we present a centrifugal microfluidic concept employing event-triggered valving for automated extraction of metered plasma and peripheral blood mononuclear cells (PBMCs). This "lab-on-a-disk"system has been developed for retrieving different density layers from a liquid column by "overflowing"the layers sequentially using the pressure exerted by a density-gradient liquid.
Defined volumes of plasma and PBMCs were efficiently forwarded into designated microfluidic chambers as a sample preparation step prior to further downstream processing. Furthermore, the extracted PBMCs were counted directly on-disk using an automated optical unit by object-based image analysis, thus eliminating the requirement for the post-processing of the extracted PBMCs.
This study is a direct continuation of our previous work1 where we demonstrated combined on-disk detection of C-reactive protein and quantification of PBMCs following on-disk extraction of plasma and PBMCs from a single blood sample using a centrifugo-pneumatic valving mechanism. However, the former valving technique featured limited PBMC extraction efficiency.
Here, integrating the novel concept along with event-triggered valving mechanism, we eliminated the occurrence of a specific microfluidic effect, which led us to increase PBMC extraction efficiency to 88%. This extraction method has the potential to be utilized for efficiently separating multiple density layers from a liquid sample in relevant biomedical applications.
Language: | English |
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Publisher: | AIP Publishing LLC |
Year: | 2021 |
Pages: | 064102 |
ISSN: | 19321058 |
Types: | Journal article |
DOI: | 10.1063/5.0066128 |
ORCIDs: | 0000-0002-0169-9023 , 0000-0003-1968-2016 , 0000-0002-0366-1897 and Boisen, Anja |