Journal article
Exploring the Inhibitory Activity of Selected Lactic Acid Bacteria against Bread Rope Spoilage Agents
National Food Institute, Technical University of Denmark1
Research Group for Gut, Microbes and Health, National Food Institute, Technical University of Denmark2
High Density Physiology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Upstream Process Development, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Research Group for Microbial Biotechnology and Biorefining, National Food Institute, Technical University of Denmark6
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark7
Technical University of Denmark8
University of Modena and Reggio Emilia9
In this study, a wide pool of lactic acid bacteria strains deposited in two recognized culture collections was tested against ropy bread spoilage bacteria, specifically belonging to Bacillus spp., Paenibacillus spp., and Lysinibacillus spp. High-throughput and ex vivo screening assays were performed to select the best candidates.
They were further investigated to detect the production of active antimicrobial metabolites and bacteriocins. Moreover, technological and safety features were assessed to value their suitability as biocontrol agents for the production of clean-label bakery products. The most prominent inhibitory activities were shown by four strains of Lactiplantibacillus plantarum (NFICC19, NFICC 72, NFICC163, and NFICC 293), two strains of Pediococcus pentosaceus (NFICC10 and NFICC341), and Leuconostoc citreum NFICC28.
Moreover, the whole genome sequencing of the selected LAB strains and the in silico analysis showed that some of the strains contain operons for bacteriocins; however, no significant evidence was observed phenotypically.
| Language: | English |
|---|---|
| Publisher: | MDPI AG |
| Year: | 2023 |
| ISSN: | 23115637 |
| Types: | Journal article |
| DOI: | 10.3390/fermentation9030290 |
| ORCIDs: | Iosca, Giovanna , Fugaban, Joanna Ivy Irorita , Özmerih, Süleyman , Wätjen, Anders Peter , Kaas, Rolf Sommer , Shetty, Radhakrishna and Bang-Berthelsen, Claus Heiner |
