Journal article
Regiospecific naphthalene monohydroxylation by a recombinant yeast producing a P4501A1–yeast reductase fused enzyme
The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, London, UK
The use of a recombinant yeast producing a P4501A1–yeast reductase fused enzyme for the regiospecific hydroxylation of naphthalene to α-naphthol is reported. During the biotransformation, significant levels of naphthalene and α-naphthol were detected in the cell fraction. Initial reaction rates of up to 23 and 13μmol/g dry cell weight/h at 5.6 and 11.2gdcw/l, respectively, were measured.
The presence of naphthalene at concentrations above aqueous phase saturation (0.45mM) as a solid was found to increase activity only marginally while the product, α-naphthol, was found to inhibit hydroxylating activity.
Language: | English |
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Year: | 2003 |
Pages: | 606-611 |
ISSN: | 18790909 and 01410229 |
Types: | Journal article |
DOI: | 10.1016/S0141-0229(03)00185-6 |