About

Log in?

DTU users get better search results including licensed content and discounts on order fees.

Anyone can log in and get personalized features such as favorites, tags and feeds.

Log in as DTU user Log in as non-DTU user No thanks

DTU Findit
Boolean: (bicycle AND helmet) OR (head AND protection) (always group AND in parenthesis)
Title: title:(climate change)
Author: author:("bohr niels" OR "bohr n") (avoid only full first name)
Phrase: "water pump control" (does not work with wildcards)
Wildcards: wom?n pharm*

More help on how to search

Flexible matching

About search syntax

Search syntax Advanced
Close advanced
{{problem}}
By field Using AND/OR By author
This tool can help you build boolean queries suitable for doing a broad search for different synonyms or spellings of each word. Start via the drop-down below or just start typing in the search field.
{{ row.typeTitle }}: ( )

Since our index often lack the full first name of people it can be difficult to correctly search by author.

Fill in the fields and let us help you build a good query:

If you need to find everything possible, consider only searching by last name.

Use "Starting year" to ignore publications out of year range.

More...

Journal article

Quantitative Proteomics Analysis of Barley-Based Liquid Feed and the Effect of Protease Inhibitors and NADPH-Dependent Thioredoxin Reductase/Thioredoxin (NTR/Trx) System

By Sultan, Abida1,2; Andersen, Birgit3; Christensen, Jesper Bjerg4; Poulsen, Hanne Damgaard4; Svensson, Birte5,6,7; Finnie, Christine3

From

Bacterial Signal Transduction, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1

Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2

Department of Systems Biology, Technical University of Denmark3

Aarhus University4

Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark5

Enzyme and Protein Chemistry, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark6

Department of Biotechnology and Biomedicine, Technical University of Denmark7

Liquid feeding strategies have been devised with the aim of enhancing grain nutrient availability for livestock. It is characterized by a steeping/soaking period that softens the grains and initiates mobilization of seed storage reserves. The present study uses 2D gel-based proteomics to investigate the role of proteolysis and reduction by thioredoxins over a 48 h steeping period by monitoring protein abundance dynamics in barley-based liquid feed samples supplemented with either protease inhibitors or NADPH-dependent thioredoxin reductase/thioredoxin (NTR/Trx).

Several full-length storage proteins were only identified in the water-extractable fraction of feed containing protease inhibitors, illustrating significant inhibition of proteolytic activities arising during the steeping period. Application of functional NTR/Trx to liquid feed reductively increased the solubility of known and potentially new Trx-target proteins, e.g., outer membrane protein X, and their susceptibility to proteolysis.

Thus, the NTR/Trx system exhibits important potential as a feed additive to enhance nutrient digestibility in monogastric animals.

Language: English
Publisher: American Chemical Society
Year: 2019
Pages: 6432-6444
ISSN: 15205118 and 00218561
Types: Journal article
DOI: 10.1021/acs.jafc.9b01708
ORCIDs: Sultan, Abida and Svensson, Birte
Keywords

NADPH-dependent thioredoxin reductase/thioredoxin system barley proteome mass spectrometry protease inhibitors

Other keywords

Animal Feed Electrophoresis, Gel, Two-Dimensional Food Handling Hordeum Plant Extracts Plant Proteins Protease Inhibitors Proteomics Seeds Thioredoxin-Disulfide Reductase Thioredoxins

DTU users get better search results including licensed content and discounts on order fees.

Log in as DTU user

Access

Analysis