Journal article · Ahead of Print article
Membrane interactions in drug delivery: Model cell membranes and orthogonal techniques
Nanofabrication, National Centre for Nano Fabrication and Characterization, Technical University of Denmark1
Polymer Microsystems, Nanofabrication, National Centre for Nano Fabrication and Characterization, Technical University of Denmark2
National Centre for Nano Fabrication and Characterization, Technical University of Denmark3
University of Copenhagen4
To generate the desired effect in the human body, the active pharmaceutical ingredient usually needs to interact with a receptor located on the cell membrane or inside the cell. Thus, understanding membrane interactions is of great importance when it comes to the development and testing of new drug molecules or new drug delivery systems.
Nowadays, there is a tremendous selection of both model cell membranes and of techniques that can be used to characterize interactions between selected model cell membranes and a drug molecule, an excipient, or a drug delivery system. Having such a wide selection of model cell membranes and techniques available makes it sometimes challenging to select the optimal combination for a specific study.
Furthermore, it is difficult to compare results obtained using different model cell membranes and techniques, and not all in vitro studies translate as well to an estimation of the in vivo biological activity or understanding of mode of action. This review provides an overview of the available lipid bilayer-based model cell membranes and of the most widely employed techniques for studying membrane interactions.
Finally, the need for employing complimentary characterization techniques in order to acquire more reliable and in-depth information is highlighted.
Language: | English |
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Year: | 2020 |
Pages: | 102177 |
ISSN: | 18733727 and 00018686 |
Types: | Journal article and Ahead of Print article |
DOI: | 10.1016/j.cis.2020.102177 |
ORCIDs: | Bunea, Ada-Ioana , Taboryski, Rafael J. , 0000-0001-9149-6061 and 0000-0002-7285-9100 |
ANTIMICROBIAL PEPTIDES Animals BIOLOGICAL-MEMBRANES CRYOELECTRON MICROSCOPY STRUCTURE Cell Membrane Drug Delivery Systems GOLD NANOPARTICLES Humans MOLECULAR-DYNAMICS SIMULATIONS Models, Biological PATCH-CLAMP PHOSPHOLIPID-VESICLES QUARTZ-CRYSTAL MICROBALANCE SUPPORTED LIPID-BILAYERS SURFACE-PLASMON RESONANCE drug delivery drug testing lipid bilayer membrane interaction