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Journal article

Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids

By Goubet, Astrid1; Chardon, Antoine; Kumar, Pawan; Sharma, Pawan K; Veedu, Rakesh N

From

School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia.1

C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields.

Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.

Language: English
Year: 2013
Pages: 761-3
ISSN: 14643405 and 0960894x
Types: Journal article
DOI: 10.1016/j.bmcl.2012.11.096
Keywords

Acetylene Base Sequence Benzenesulfonamides DNA-Directed DNA Polymerase Molecular Sequence Data Molecular Structure Nucleic Acids Nucleotides Oligonucleotides Polymerase Chain Reaction Substrate Specificity Sulfonamides

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