Journal article
Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids
School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia.1
C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields.
Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.
Language: | English |
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Year: | 2013 |
Pages: | 761-3 |
ISSN: | 14643405 and 0960894x |
Types: | Journal article |
DOI: | 10.1016/j.bmcl.2012.11.096 |