Journal article
A simple hairpin DNA sensor for label-free detection of sub-attomole DNA target
College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China1
State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China2
In this paper, we explored a label-free, ultrasensitive and simple method of DNA detection based on hairpin DNA via a high-resolution surface plasmon resonance (SPR). Using this simple direct hybridization system, a detection level of 0.5fM was obtained and the reason of the high sensitivity was discussed.
Moreover, the prepared DNA sensor could also discriminate single-base mismatched target and was amenable to study the effect of mismatch position in target DNA. From the results, it can be seen that mutation in the center of target DNA had a greater influence on the hybridization. Then, a simple, inexpensive, high sensitive and excellent selective DNA sensor was developed.
| Language: | English |
|---|---|
| Year: | 2010 |
| Pages: | 561-565 |
| ISSN: | 18733077 and 09254005 |
| Types: | Journal article |
| DOI: | 10.1016/j.snb.2010.03.059 |
