Journal article
Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, Denmark.1
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions.
Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.
Language: | English |
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Publisher: | The Royal Society of Chemistry |
Year: | 2009 |
Pages: | 1404-1409 |
ISSN: | 14770539 and 14770520 |
Types: | Journal article |
DOI: | 10.1039/b819946a |