Journal article
Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase
In
Organic and Biomolecular Chemistry
—
2009, Volume 7, Issue 7, pp. 1404-1409
From
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, Denmark.1
Abstract
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions.
Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.
| Language: | English |
|---|---|
| Publisher: | The Royal Society of Chemistry |
| Year: | 2009 |
| Pages: | 1404-1409 |
| ISSN: | 14770539 and 14770520 |
| Types: | Journal article |
| DOI: | 10.1039/b819946a |
