Journal article
CD103+ CD11b+ mucosal classical dendritic cells initiate long-term switched antibody responses to flagellin
University of Birmingham1
Lund University2
Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
Mucosal Immunology, Division of Immunology & Vaccinology, National Veterinary Institute, Technical University of Denmark5
Antibody responses induced at mucosal and nonmucosal sites demonstrate a significant level of autonomy. Here, we demonstrate a key role for mucosal interferon regulatory factor-4 (IRF4)-dependent CD103+ CD11b+(DP), classical dendritic cells (cDCs) in the induction of T-dependent immunoglobulin G (IgG) and immunoglobulin A (IgA) responses in the mesenteric lymph node (MLN) following systemic immunization with soluble flagellin (sFliC).
In contrast, IRF8-dependent CD103+ CD11b- (SP) are not required for these responses. The lack of this response correlated with a complete absence of sFliC-specific plasma cells in the MLN, small intestinal lamina propria, and surprisingly also the bone marrow (BM). Many sFliC-specific plasma cells accumulating in the BM of immunized wild-type mice expressed α4β 7+, suggesting a mucosal origin.
Collectively, these results suggest that mucosal DP cDC contribute to the generation of the sFliC-specific plasma cell pool in the BM and thus serve as a bridge linking the mucosal and systemic immune system.
Language: | English |
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Publisher: | Nature Publishing Group US |
Year: | 2018 |
Pages: | 681-692 |
Journal subtitle: | Official Publication of the Society for Mucosal Immunology |
ISSN: | 19353456 and 19330219 |
Types: | Journal article |
DOI: | 10.1038/mi.2017.105 |
ORCIDs: | Agace, W. W. |
Animals Antigens, CD CD11b Antigen Cells, Cultured Dendritic Cells Flagellin Immunity, Humoral Immunoglobulin A Immunoglobulin Class Switching Immunoglobulin G Integrin alpha Chains Integrin alpha4 Integrin beta Chains Interferon Regulatory Factors Lymph Nodes Mice Mice, Inbred C57BL Mice, Knockout Mucous Membrane Plasma Cells alpha E integrins integrin beta7 interferon regulatory factor-4 interferon regulatory factor-8