Journal article
High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium
Nannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis salina biomass, with a focus on eicosapentaenoic acid (EPA).
Variations in fatty acid composition, lipids, protein, amino acids, tocopherols and pigments were studied and results compared to algae cultivated on F/2 media as reference. Mixed growth media and process water enhanced the nutritional quality of Nannochloropsis salina in laboratory scale when compared to algae cultivated in standard F/2 medium.
Data from laboratory scale translated to the large scaleusing a 4000 L flat panel photo-bioreactor system. The algae growth rate in winter conditions in Denmark was slow, but results revealed that large-scale cultivation of Nannochloropsis salina at these conditions could improve the nutritional properties such as EPA, tocopherol, protein and carotenoids compared to laboratory-scale cultivated microalgae.
EPA reached 44.2% ± 2.30% of total fatty acids, and α-tocopherol reached 431 ± 28 µg/g of biomass dry weight after 21 days of cultivation. Variations in chemical compositions of Nannochloropsis salina were studied during the course of cultivation. Nannochloropsis salina can be presented as a good candidate for winter time cultivation in Denmark.The resulting biomass is a rich source of EPA and also a good source of protein (amino acids), tocopherols and carotenoids for potential use in aquaculture feed industry.
Language: | English |
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Publisher: | MDPI |
Year: | 2016 |
Pages: | 144 |
ISSN: | 16603397 |
Types: | Journal article |
DOI: | 10.3390/md14080144 |
ORCIDs: | Safafar, Hamed , Holdt, Susan Løvstad and Jacobsen, Charlotte |
Amino acid Carotenoids EPA Flat panel photo-bioreactor Industrial process water Large scale Microalgae Nannochloropsis salina Tocopherol
Animal Feed Aquaculture Batch Cell Culture Techniques Biofuels Biology (General) Bioreactors Culture Media Eicosapentaenoic Acid Light QH301-705.5 Stramenopiles Temperature Time Factors Water alpha-Tocopherol amino acid carotenoids flat panel photo-bioreactor industrial process water large scale microalgae tocopherol