Journal article
Design of enzymatic cascade processes for the production of low-priced chemicals
Universidad Nacional de Colombia1
PROSYS - Process and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark2
KT Consortium, Department of Chemical and Biochemical Engineering, Technical University of Denmark3
Department of Chemical and Biochemical Engineering, Technical University of Denmark4
While the application of enzymes to synthetic and industrial problems continues to grow, the major development today is focused on multi-enzymatic cascades. Such systems are particularly attractive, because many commercially available enzymes operate under relatively similar operating conditions. This opens the possibility of one-pot operation with multiple enzymes in a single reactor.
In this paper the concept of modules is introduced whereby groups of enzymes are combined in modules, each operating in a single reactor, but with the option of various operating strategies to avoid any complications of nonproductive interactions between the enzymes, substrates or products in a given reactor.
In this paper the selection of modules is illustrated using the synthesis of the bulk chemical, gluconic acid, from lignocellulosic waste.
| Language: | English |
|---|---|
| Year: | 2019 |
| Pages: | 77-84 |
| ISSN: | 09395075 , 18657125 and 03410382 |
| Types: | Journal article |
| DOI: | 10.1515/znc-2018-0190 |
| ORCIDs: | Woodley, John M. |
Biocatalysis Catalase Cellulases Computer Simulation Fermentation Gluconates Glucose Glucose Oxidase Humans Hydrogen-Ion Concentration Kinetics Lactones Lignin Metabolic Engineering Models, Statistical Temperature Waste Products beta-Glucosidase beta-glucono-1,5-lactone biocatalyst biocatalytic reactions gluconic acid lignocellulose multi-enzymatic cascade reaction modules
