Journal article
Antimicrobial peptide CAP18 and its effect on Yersinia ruckeri infections in rainbow trout Oncorhynchus mykiss (Walbaum): comparing administration by injection and oral routes
University of Copenhagen1
National Food Institute, Technical University of Denmark2
Research Group for Gut Microbiology and Immunology, National Food Institute, Technical University of Denmark3
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark4
Aalborg University5
BioMar A/S6
National Veterinary Institute, Technical University of Denmark7
Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark8
The antimicrobial peptide CAP18 has been demonstrated to have a strong in vitro bactericidal effect on Yersinia ruckeri, but its activity in vivo has not been described. In this work, we investigated whether CAP18 protects rainbow trout Oncorhynchus mykiss (Walbaum) against enteric red mouth disease caused by this pathogen either following i.p. injection or by oral administration (in feed).
It was found that injection of CAP18 into juvenile rainbow trout before exposure to Y. ruckeri was associated with lowered mortality compared to non-medicated fish although it was less effective than the conventional antibiotic oxolinic acid. Oral administration of CAP18 to trout did not prevent infection.
The proteolytic effect of secretions on the peptide CAP18 in the fish gastrointestinal tract is suggested to account for the inferior effect of oral administration.
| Language: | English |
|---|---|
| Year: | 2017 |
| Pages: | 97-104 |
| ISSN: | 13652761 and 01407775 |
| Types: | Journal article |
| DOI: | 10.1111/jfd.12497 |
| ORCIDs: | 0000-0002-2747-237X , Hansen, Egon Bech and Dalsgaard, Inger |
