Journal article
Structure dependent antioxidant capacity of phlorotannins from Icelandic Fucus vesiculosus by UHPLC-DAD-ECD-QTOFMS
National Food Institute, Technical University of Denmark1
Research Group for Bioactives – Analysis and Application, National Food Institute, Technical University of Denmark2
Lund University3
Matís4
Department of Biotechnology and Biomedicine, Technical University of Denmark5
DTU Metabolomics Core, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark6
Brown algae are rich in polyphenolic compounds, phlorotannins, which have been found to possess high in vitro antioxidant capacity, especially DPPH radical scavenging activity, due to the high number of hydroxyl groups. Whereas, the overall antioxidant capacity of brown algae extracts has been widely studied, the antioxidant capacity of individual phlorotannins has been rarely explored.
The aim of this study was to determine the structure dependant antioxidant capacity of phlorotannins from Icelandic brown algae, Fucus vesiculosus. The antioxidant capacity of individual phlorotannins was determined by an on-line method using liquid chromatography and an electrochemical detector followed by quadrupole Time of Flight mass spectrometry (UHPLC-DAD-ECD-QTOFMS).
Tentative structural elucidation of 13 phlorotannin isomers from EAF was obtained by LC-DAD-QTOFMS, ranging from 374 to 870 Da. On-line determination of antioxidant capacity of the individual phlorotannins generally showed that low molecular phlorotannins exhibited higher antioxidant capacity and that the capacity decreased with polymerisation.
Language: | English |
---|---|
Year: | 2018 |
Pages: | 904-909 |
ISSN: | 03088146 and 18737072 |
Types: | Journal article |
DOI: | 10.1016/j.foodchem.2017.08.032 |
ORCIDs: | 0000-0002-9636-6458 , Hermund, Ditte Baun , Jacobsen, Charlotte and Nielsen, Kristian Fog |