Journal article
Explorative Solid-Phase Extraction (E-SPE) for Accelerated Microbial Natural Product Discovery, Dereplication, and Purification
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark1
Department of Systems Biology, Technical University of Denmark2
Division of Seafood Research, National Food Institute, Technical University of Denmark3
National Food Institute, Technical University of Denmark4
Microbial natural products (NP) cover a high chemical diversity, and in consequence extracts from microorganisms are often complex to analyze and purify. A distribution analysis of calculated pK(a) values from the 34390 records in Antibase2008 revealed that within pH 2-11, 44% of all included compounds had an acidic functionality, 17% a basic functionality, and 9% both.
This showed a great potential for using ion-exchange chromatography as an integral part of the separation procedure, orthogonal to the classic reversed-phase strategy. Thus, we investigated the use of an "explorative solid-phase extraction" (E-SPE) protocol using SAX, Oasis MAX, SCX, and LH-20 columns for targeted exploitation of chemical functionalities.
E-SPE provides a minimum of fractions (15) for chemical and biological analyses and implicates development into a preparative scale methodology. Overall, this allows fast extract prioritization, easier dereplication, mapping of biological activities, and formulation of a purification strategy.
Language: | English |
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Year: | 2010 |
Pages: | 1126-1132 |
ISSN: | 15206025 and 01633864 |
Types: | Journal article |
DOI: | 10.1021/np100151y |
ORCIDs: | Gram, Lone , Larsen, Thomas Ostenfeld and Nielsen, Kristian Fog |