Journal article
Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark4
National Food Institute, Technical University of Denmark5
Secretariat, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark6
A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen.
The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype.
The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).
Language: | English |
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Year: | 2001 |
Pages: | 11-18 |
ISSN: | 18732542 and 03781135 |
Types: | Journal article |
DOI: | 10.1016/S0378-1135(00)00349-7 |
ORCIDs: | Andresen, Lars Ole |