Journal article
Dynamic in situ chromosome immobilisation and DNA extraction using localized poly(N-isopropylacrylamide) phase transition
NSE-Optofluidics Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Nanoprobes, Department of Micro- and Nanotechnology, Technical University of Denmark4
The Danish Polymer Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark5
Department of Chemical and Biochemical Engineering, Technical University of Denmark6
A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel.
It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel. © 2011 American Institute of Physics.
| Language: | English |
|---|---|
| Publisher: | American Institute of Physics |
| Year: | 2011 |
| Pages: | 031101-031101-4 |
| ISSN: | 19321058 |
| Types: | Journal article |
| DOI: | 10.1063/1.3637631 |
| ORCIDs: | Marie, Rodolphe , Svendsen, Winnie Edith , Szabo, Peter and Kristensen, Anders |
