Journal article
Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: Effects on liver DNA strand breaks in dams and offspring
Roskilde University1
National Research Centre for the Working Environment2
Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark3
National Food Institute, Technical University of Denmark4
University of Copenhagen5
Norwegian Institute of Public Health6
Effects of maternal pulmonary exposure to carbon black (Printex 90) on gestation, lactation and DNA strand breaks were evaluated. Time-mated C57BL/6BomTac mice were exposed by inhalation to 42 mg/m3 Printex 90 for 1 h/day on gestation days (GD) 8–18, or by four intratracheal instillations on GD 7, 10, 15 and 18, with total doses of 11, 54 and 268 mg/animal.
Dams were monitored until weaning and some offspring until adolescence. Inflammation was assessed in maternal bronchoalveolar lavage (BAL) 3–5 days after exposure, and at weaning. Levels of DNA strand breaks were assessed in maternal BAL cells and liver, and in offspring liver. Persistent lung inflammation was observed in exposed mothers.
Inhalation exposure induced more DNA strand breaks in the liver of mothers and their offspring, whereas intratracheal instillation did not. Neither inhalation nor instillation affected gestation and lactation. Maternal inhalation exposure to Printex 90-induced liver DNA damage in the mothers and the in utero exposed offspring.
| Language: | English |
|---|---|
| Publisher: | Informa Healthcare |
| Year: | 2011 |
| Pages: | 486-500 |
| ISSN: | 17435404 and 17435390 |
| Types: | Journal article |
| DOI: | 10.3109/17435390.2011.587902 |
| ORCIDs: | 0000-0002-2021-1249 and 0000-0001-9552-8518 |
Administration, Inhalation Animals Bronchoalveolar Lavage Fluid Carbon black Chemical and Drug Induced Liver Injury DNA Damage Drug-Induced Liver Injury Female Inhalation Exposure Lactation Liver Male Maternal Exposure Mice Mice, Inbred C57BL Nanoparticles Organ Size Pneumonia Pregnancy Soot genotoxicity gestation and lactation in utero exposure inflammation nanoparticles pulmonary exposure
