Conference paper
Optical and hydrodynamic stretching of single cells from blood
Technical University of Denmark1
Copenhagen University Hospital Herlev and Gentofte2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Optofluidics, Department of Micro- and Nanotechnology, Technical University of Denmark4
Department of Physics, Technical University of Denmark5
Quantum Physics and Information Technology, Department of Physics, Technical University of Denmark6
Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor.
In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step.
Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.
Language: | English |
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Publisher: | The Optical Society (OSA) |
Year: | 2017 |
Proceedings: | OSA Biophotonics Congress: Optical Trapping Applications 2017 |
ISBN: | 1557528209 and 9781557528209 |
ISSN: | 21622701 |
Types: | Conference paper |
DOI: | 10.1364/OTA.2017.OtM4E.1 |
ORCIDs: | 0000-0001-8034-1523 , Kristensen, Anders , Marie, Rodolphe and Berg-Sørensen, Kirstine |