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Journal article

Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid-liquid extraction and surface enhanced Raman scattering

By Morelli, Lidia1,2; Serioli, Laura1,2; Centorbi, Francesca Alessandra3; Jendresen, Christian Bille4,5; Matteucci, Marco1,2; Ilchenko, Oleksii1,2; Demarchi, Danilo3; Nielsen, Alex Toftgaard4,5,6; Zor, Kinga1,2; Boisen, Anja1,2

From

Department of Micro- and Nanotechnology, Technical University of Denmark1

Nanoprobes, Department of Micro- and Nanotechnology, Technical University of Denmark2

Polytechnic University of Turin3

Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4

Bacterial Cell Factory Optimization, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5

Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6

Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark7

We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding.

The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.

Language: English
Publisher: The Royal Society of Chemistry
Year: 2018
Pages: 869-877
ISSN: 14730189 and 14730197
Types: Journal article
DOI: 10.1039/c7lc01217a
ORCIDs: Jendresen, Christian Bille , Ilchenko, Oleksii , Nielsen, Alex Toftgaard , Zor, Kinga , Boisen, Anja , 0000-0002-3299-8608 , 0000-0003-3502-0178 , 0000-0002-3660-5621 and 0000-0001-5374-1679
Keywords

Chromatography, High Pressure Liquid Coumaric Acids Escherichia coli Liquid-Liquid Extraction Microfluidic Analytical Techniques Polypropylenes Propionates Spectrum Analysis, Raman Surface Properties p-coumaric acid

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