Journal article
Multi-particle three-dimensional coordinate estimation in real-time optical manipulation
Teraherts Technologies and Biophotonics, Department of Photonics Engineering, Technical University of Denmark1
Department of Photonics Engineering, Technical University of Denmark2
BioLabChip Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark3
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Department of Micro- and Nanotechnology, Technical University of Denmark5
We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration.
Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.
Language: | English |
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Year: | 2009 |
ISSN: | 19902573 |
Types: | Journal article |
DOI: | 10.2971/jeos.2009.09045 |