Journal article
Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1
The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests.
Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1.
Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.
Language: | English |
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Year: | 2018 |
Pages: | 196-202 |
ISSN: | 1878562x and 15675394 |
Types: | Journal article |
DOI: | 10.1016/j.bioelechem.2017.10.005 |