Journal article
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
BGI Group1
GT Life Sciences2
Shenzhen Hospital3
University of Delaware4
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark5
Department of Systems Biology, Technical University of Denmark6
Stanford University7
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark8
Network Reconstruction in Silico Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark9
Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes.
Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions.
Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production.
Language: | English |
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Publisher: | Nature Publishing Group |
Year: | 2011 |
Pages: | 735-741 |
Journal subtitle: | Science and Business of Biotechnology |
ISSN: | 15461696 and 10870156 |
Types: | Journal article |
DOI: | 10.1038/nbt.1932 |
ORCIDs: | Andersen, Mikael Rørdam and Palsson, Bernhard |