Journal article
Cutaneous immune responses in the common carp detected using transcript analysis
Department of Veterinary Pathobiology, Laboratory for Fish Diseases, The Royal Veterinary and Agricultural University, Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark. xanti@usc.es1
In order to detect new immune-related genes in common carp (Cyprinus carpio L.) challenged by an ectoparasitic infection, two cDNA libraries were constructed from carp skin sampled at 3 and 72h after infection with Ichthyophthirius multifiliis. In a total of 3500 expressed sequence tags (ESTs) we identified 82 orthologues of genes of immune relevance previously described in other organisms.
Of these, 61 have never been described before in C. carpio, thus shedding light on some key components of the defence mechanisms of this species. Among the newly described genes, full-length molecules of prostaglandin D2 synthase (PGDS), the CC chemokine molecule SCYA103, and a second gene for the carp beta(2)-microglobulin (beta(2)m), beta(2)m-2, were described.
Transcript amounts of the genes PGDS, interferon (IFN), SCYA103, complement factor 7 (C7), complement factor P (FP), complement factor D (FD) and beta(2)m-2 were evaluated by real-time quantitative PCR (RQ-PCR). Samples from skin, blood and liver from fish challenged with I. multifiliis were taken at 3, 12, 24, 36 and 48h post infection.
Higher expression levels of most of these transcripts were observed in skin from uninfected fish, compared to the transcript levels detected in blood and liver from the same animals. Also, there was significant down-regulation of the genes PGDS and beta(2)m-2 in skin, whilst significant up-regulation was observed for the C7 and SCYA103 genes in liver of fish infected with the parasite.
These results confirm the active role of fish skin in the immune response against infections, acting as an important site of expression of immune-related molecules.
Language: | English |
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Year: | 2007 |
Pages: | 1664-1679 |
ISSN: | 18729142 and 01615890 |
Types: | Journal article |
DOI: | 10.1016/j.molimm.2006.08.016 |
Amino Acid Sequence Animals Antigen Presentation Beta 2 microglobulin CC chemokine Carps Chemotaxis Complement Complement System Proteins Cyprinus carpio Expressed Sequence Tags Expressed sequence tag Ichthyophthirius multifiliis Immunity Immunity, Innate Inflammation Interferon Molecular Sequence Data Prostaglandin D synthase RNA, Messenger Real-time PCR Signal Transduction Skin Transcription, Genetic