Journal article
Development of novel monoclonal antibodies against starch and ulvan - Implications for antibody production against polysaccharides with limited immunogenicity
University of Copenhagen1
Newcastle University2
Carlsberg Research Center3
Sorbonne Université4
Department of Chemical and Biochemical Engineering, Technical University of Denmark5
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark6
Department of Biotechnology and Biomedicine, Technical University of Denmark7
Enzyme and Protein Chemistry, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark8
INRAE9
Skidmore College10
...and 0 moreMonoclonal antibodies (mAbs) are widely used and powerful research tools, but the generation of mAbs against glycan epitopes is generally more problematic than against proteins. This is especially significant for research on polysaccharide-rich land plants and algae (Viridiplantae). Most antibody production is based on using single antigens, however, there are significant gaps in the current repertoire of mAbs against some glycan targets with low immunogenicity.
We approached mAb production in a different way and immunised with a complex mixture of polysaccharides. The multiplexed screening capability of carbohydrate microarrays was then exploited to deconvolute the specificities of individual mAbs. Using this strategy, we generated a set of novel mAbs, including one against starch (INCh1) and one against ulvan (INCh2).
These polysaccharides are important storage and structural polymers respectively, but both are generally considered as having limited immunogenicity. INCh1 and INCh2 therefore represent important new molecular probes for Viridiplantae research. Moreover, since the α-(1-4)-glucan epitope recognised by INCh1 is also a component of glycogen, this mAb can also be used in mammalian systems.
We describe the detailed characterisation of INCh1 and INCh2, and discuss the potential of a non-directed mass-screening approach for mAb production against some glycan targets.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2017 |
Pages: | 9326 |
ISSN: | 20452322 |
Types: | Journal article |
DOI: | 10.1038/s41598-017-04307-2 |
ORCIDs: | 0000-0002-9388-0873 , 0000-0002-5795-1320 , Wilkens, Casper , Svensson, Birte , 0000-0003-0331-3304 , 0000-0001-5582-9463 , 0000-0002-0453-1277 and 0000-0003-2064-4025 |