Journal article
Feruloylated and Nonferuloylated Arabino-oligosaccharides from Sugar Beet Pectin Selectively Stimulate the Growth of Bifidobacterium spp. in Human Fecal in Vitro Fermentations
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Department of Chemical and Biochemical Engineering, Technical University of Denmark2
University of Southern Denmark3
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark4
National Food Institute, Technical University of Denmark5
The side chains of the rhamnogalacturonan I fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. In this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by Amberlite XAD hydrophobic interaction and membrane separation into four fractions based on feruloyl substitution and arabino-oligosaccharide chain length: short-chain (DP 2–10) and long-chain (DP 7–14) feruloylated and nonferuloylated arabino-oligosaccharides, respectively.
HPAEC, SEC, and MALDI-TOF/TOF analyses of the fractions confirmed the presence of singly and doubly substituted feruloylated arabino-oligosaccharides in the feruloyl-substituted fractions. In vitro microbial fermentation by human fecal samples (n = 6 healthy human volunteers) showed a selective stimulation of bifidobacteria by both the feruloylated and the nonferuloylated long-chain arabino-oligosaccharides to the same extent as the prebiotic fructo-oligosaccharides control.
None of the fractions stimulated the growth of the potential pathogen Clostridium difficile in monocultures. This work provides a first report on the separation of potentially bioactive feruloylated arabino-oligosaccharides from sugar beet pulp and an initial indication of the potentially larger bifidogenic effect of relatively long-chain arabino-oligosaccharides as opposed to short-chain arabino-oligosaccharides.
Language: | English |
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Year: | 2011 |
Pages: | 6511-6519 |
ISSN: | 15205118 and 00218561 |
Types: | Journal article |
DOI: | 10.1021/jf200996h |
ORCIDs: | Holck, Jesper , Licht, Tine Rask , Mikkelsen, Jørn Dalgaard and Meyer, Anne S. |