Journal article
Morphological and molecular characterisation of Eimeria vison-like oocysts (Apicomplexa:Eimeriidae) in farmed mink (Neovison vison) in Denmark
National Veterinary Institute, Technical University of Denmark1
Bacteriology & Parasitology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark2
Murdoch University3
Diagnostic & Development, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark4
Pathology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark5
A survey was conducted on 30 Danish mink farms from April to October 2016 to determine the prevalence and species of Eimeria in Danish farmed mink. In total, 2.6% of mink faecal samples (108/4140) were positive for Eimeria vison-like oocysts by microscopy, with 24.8% (78/315) of mink being positive at least once during the study period.
Morphological analysis of sporulated oocysts (n = 20) identified Eimeria vison-like oocysts measuring 21.0 × 13.8 μm with a length/width (L/W) ratio of 1.5. Phylogenetic analysis of 18S rRNA sequences (1221 bp) from three positive mink indicated that Eimeria vison-like shared the highest genetic similarity to Eimeria sp. ex Apodemus agrarius from a Striped field mouse (A. agrarius) from the Czech Republic (99.6%).
Analysis of a shorter region of 18S (531 bp) revealed that the E. vison-like genotype sequences grouped in the same clade and shared 97.7% similarity with E. furonis. At the cytochrome c oxidase subunit I (COI) locus, mink-derived sequences were not available from GenBank and phylogenetic analysis placed the novel E. vison-like in a clade with E. cf. ictidea (99.4% similarity) from a black footed ferret (Mustela nigripes) from Canada.
Language: | English |
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Publisher: | Springer Berlin Heidelberg |
Year: | 2018 |
Pages: | 2933-2939 |
Journal subtitle: | Founded As Zeitschrift Für Parasitenkunde |
ISSN: | 14321955 and 09320113 |
Types: | Journal article |
DOI: | 10.1007/s00436-018-5989-1 |
ORCIDs: | Petersen, H. H. , Chriél, M. and Hansen, M. S. |