Journal article
Macrophage and dendritic cell subsets in IBD: ALDH+ cells are reduced in colon tissue of patients with ulcerative colitis regardless of inflammation
University of Gothenburg1
Aarhus University Hospital2
Lund University3
Sahlgrenska University Hospital4
Skåne University Hospital5
National Veterinary Institute, Technical University of Denmark6
Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark7
Disruption of the homeostatic balance of intestinal dendritic cells (DCs) and macrophages (MQs) may contribute to inflammatory bowel disease. We characterized DC and MQ populations, including their ability to produce retinoic acid, in clinical material encompassing Crohn’s ileitis, Crohn’s colitis and ulcerative colitis (UC) as well as mesenteric lymph nodes (MLNs) draining these sites.
Increased CD14+DRint MQs characterized inflamed intestinal mucosa while total CD141+ or CD1c+ DCs numbers were unchanged. However, CD103+ DCs, including CD141+CD103+ and CD1c+CD103+ DCs, were reduced in inflamed intestine. In MLNs, two CD14− DC populations were identified: CD11cintHLADRhi and CD11chiHLADRint cells.
A marked increase of CD11chiHLADRint DC, particularly DRintCD1c+ DCs, characterized MLNs draining inflamed intestine. The fraction of DC and MQ populations expressing aldehyde dehydrogenase (ALDH) activity, reflecting retinoic acid synthesis, in UC colon, both in active disease and remission, were reduced compared to controls and inflamed Crohn’s colon.
In contrast, no difference in the frequency of ALDH+ cells among blood precursors was detected between UC patients and non-inflamed controls. This suggests that ALDH activity in myeloid cells in the colon of UC patients, regardless of whether the disease is active or in remission, is influenced by the intestinal environment.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group US |
| Year: | 2016 |
| Pages: | 171-182 |
| Journal subtitle: | Official Publication of the Society for Mucosal Immunology |
| ISSN: | 19353456 and 19330219 |
| Types: | Journal article |
| DOI: | 10.1038/mi.2015.48 |
| ORCIDs: | Agace, William Winston |
Adolescent Adult Aged Aged, 80 and over Aldehyde Dehydrogenase Antigens, CD Antigens, CD1 Antigens, CD11c Antigens, CD14 Antigens, Surface CD11c Antigen CD1C protein, human Case-Control Studies Colitis, Ulcerative Colon Crohn Disease Dendritic Cells Female Gene Expression Regulation Glycoproteins HLA-DR Antigens Humans Integrin alpha Chains Intestinal Mucosa Journal Article Lipopolysaccharide Receptors Lymph Nodes Macrophages Male Middle Aged Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Severity of Illness Index Signal Transduction THBD protein, human Thrombomodulin alpha E integrins
