Journal article
Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples
Central Veterinary Institute1
Federal Veterinary and Agrochemical Research Centre2
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail3
Animal Health and Veterinary Laboratories Agency4
National Veterinary Institute5
National Veterinary Institute, Technical University of Denmark6
Section for Virology, National Veterinary Institute, Technical University of Denmark7
Animal Health Service8
Friedrich-Loeffler-Institute9
Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories.
All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.
Language: | English |
---|---|
Publisher: | BMJ Group Group Ltd |
Year: | 2014 |
Pages: | 380 |
ISSN: | 20427670 , 02143909 and 00424900 |
Types: | Journal article |
DOI: | 10.1136/vr.102180 |