Journal article
High frequency RNA recombination in porcine reproductive and respiratory syndrome virus occurs preferentially between parental sequences with high similarity
Stald/vægterservice, Division of Virology, National Veterinary Institute, Technical University of Denmark1
Division of Virology, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark4
Two types of porcine reproductive and respiratory syndrome virus (PRRSV) exist, a North American type and a European type. The co-existence of both types in some countries, such as Denmark, Slovakia and Canada, creates a risk of inter-type recombination. To evaluate this risk, cell cultures were co-infected with either a North American and a European type of PRRSV or two diverse types of European isolate.
Subsequently, an approximately 600 bp region of the PRRSV genome was tested for recombination by quantitative real-time RT-PCR. Between 0-1 and 2-5% RNA recombination was found between the European isolates, but no recombination was detected between the European and North American types. Calculation of the maximum theoretical risk of European-American recombination, based on the sensitivity of the RT-PCR system, revealed that RNA recombination between the European and North American types of PRRSV is at least 10000 times less likely to occur than RNA recombination between diverse European isolates.
Language: | English |
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Publisher: | Microbiology Society |
Year: | 2001 |
Pages: | 2615-2620 |
ISSN: | 14652099 and 00221317 |
Types: | Journal article |
DOI: | 10.1099/0022-1317-82-11-2615 |