Journal article
TRAV1-2+ CD8+ T-cells including oligoconal expansions of MAIT cells are enriched in the airways in human tuberculosis
University of Copenhagen1
Immunoinformatics and Machine Learning, Bioinformatics, Department of Health Technology, Technical University of Denmark2
Bioinformatics, Department of Health Technology, Technical University of Denmark3
Department of Health Technology, Technical University of Denmark4
Mucosal-associated invariant T (MAIT) cells typically express a TRAV1-2+ semi-invariant TCRα that enables recognition of bacterial, mycobacterial, and fungal riboflavin metabolites presented by MR1. MAIT cells are associated with immune control of bacterial and mycobacterial infections in murine models.
Here, we report that a population of pro-inflammatory TRAV1-2+ CD8+ T cells are present in the airways and lungs of healthy individuals and are enriched in bronchoalveolar fluid of patients with active pulmonary tuberculosis (TB). High-throughput T cell receptor analysis reveals oligoclonal expansions of canonical and donor-unique TRAV1-2+ MAIT-consistent TCRα sequences within this population.
Some of these cells demonstrate MR1-restricted mycobacterial reactivity and phenotypes suggestive of MAIT cell identity. These findings demonstrate enrichment of TRAV1-2+ CD8+ T cells with MAIT or MAIT-like features in the airways during active TB and suggest a role for these cells in the human pulmonary immune response to Mycobacterium tuberculosis.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2019 |
| Pages: | 203 |
| ISSN: | 23993642 |
| Types: | Journal article |
| DOI: | 10.1038/s42003-019-0442-2 |
| ORCIDs: | 0000-0003-4755-5380 , 0000-0003-2505-3291 , 0000-0002-9709-7624 , 0000-0003-4482-5955 , 0000-0002-6111-6512 , 0000-0002-7021-5934 , 0000-0002-9856-2938 , 0000-0001-9416-2737 , 0000-0002-3374-6699 , Nielsen, Morten , 0000-0001-9906-9494 and 0000-0002-2165-3313 |
Animals Bronchi Bronchoalveolar Lavage Fluid Bronchoscopy CD8-Positive T-Lymphocytes Histocompatibility Antigens Class I Humans Immune System Inflammation Intestines Lung Mice Minor Histocompatibility Antigens Mucosal immunology Mucosal-Associated Invariant T Cells Mycobacterium tuberculosis Oregon Phenotype Receptors, Antigen, T-Cell, alpha-beta South Africa T-cell receptor Tuberculosis Tuberculosis, Pulmonary
