Journal article
Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics
The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia inhibition during AD.
This study aimed to achieve an efficient AD of OFMSW under extreme ammonia levels and elucidate the dynamics of the acclimatized microbial community. Thus, two mesophilic continuous stirred tank reactors (CSTR), fed only with OFMSW, were successfully acclimatized up to 8.5âŻg NH4+-N/L, and their methane yields fluctuated <10%, compared to the methane yields without ammonia addition.
Microbiological analyses showed that Methanosaeta concilii and Methanosarcina soligelidi were the dominant methanogens at low and high ammonia levels, respectively. Whilst, a unique metabolic pathway shift, from aceticlastic to hydrogenotrophic methanogenesis, of M. soligelidi was identified during the acclimatization process.
| Language: | English |
|---|---|
| Year: | 2019 |
| Pages: | 121376 |
| ISSN: | 18732976 and 09608524 |
| Types: | Journal article |
| DOI: | 10.1016/j.biortech.2019.121376 |
| ORCIDs: | Yan, Miao , Fotidis, Ioannis , Tian, Hailin , Tsapekos, Panagiotis , Angelidaki, Irini , 0000-0003-0236-5970 and 0000-0002-5053-4452 |
Aceticlastic pathway Ammonia acclimatization Hydrogenotrophic pathway Metabolic pathway shift Methanosarcina soligelidi SDG 11 - Sustainable Cities and Communities SDG 12 - Responsible Consumption and Production
Acclimatization Ammonia Anaerobiosis Bioreactors Methane Microbiota Solid Waste
