Journal article
Poor Invasion of Trophoblastic Cells but Normal Plaque Formation in Fibroblastic Cells despite actA Deletion in a Group of Listeria monocytogenes Strains Persisting in Some Food Processing Environments
We determined mammalian cell invasion and virulence gene (inlA, inlB, and actA) sequences of Listeria monocytogenes strains belonging to a molecular subtype (RAPD 9) that often persists in Danish fish-processing plants. These strains invaded human placental trophoblasts less efficiently than other L. monocytogenes strains, including clinical strains, and they carry a premature stop codon in inlA.
Eight of 15 strains, including the RAPD 9 and maternofetal strains, had a 105-nucleotide deletion in actA that did not affect cell-to-cell spread in mouse fibroblasts. The RAPD 9 strains may still be regarded as of low virulence with respect to human listeriosis.
| Language: | English |
|---|---|
| Publisher: | American Society for Microbiology (ASM) |
| Year: | 2010 |
| Pages: | 3391-3397 |
| ISSN: | 10985336 and 00992240 |
| Types: | Journal article |
| DOI: | 10.1128/AEM.02862-09 |
| ORCIDs: | Gram, Lone , 0000-0001-6242-6937 and 0000-0002-8350-5631 |
Amino Acid Sequence Animals Bacterial Proteins Cell Line Cell Line, Tumor Female Fibroblasts Food Microbiology Food-Processing Industry Gene Deletion Humans Listeria monocytogenes Listeriosis Membrane Proteins Mice Molecular Sequence Data Random Amplified Polymorphic DNA Technique Sequence Alignment Trophoblasts Virulence actA protein, Listeria monocytogenes inlB protein, Listeria monocytogenes internalin protein, Bacteria
