Journal article
Use of high-gradient magnetic fishing for reducing proteolysis during fermentation
Department of Systems Biology, Technical University of Denmark1
Center for Mikrobial Biotechnology, Technical University of Denmark2
Department of Chemical and Biochemical Engineering, Technical University of Denmark3
CHEC Research Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark4
National Food Institute, Technical University of Denmark5
Division of Industrial Food Research, National Food Institute, Technical University of Denmark6
Proteolysis during fermentation may have a severe impact on the yield and quality of a secreted product. In the current study, we demonstrate the use of high-gradient magnetic fishing (HGMF) as an efficient alternative to the more conventional methods of preventing proteolytic degradation. Bacitracin-linked magnetic affinity adsorbents were employed directly in a fermenter during Bacillus licheniformis cultivation to remove trace amounts of unwanted proteases.
The constructed magnetic adsorbents had excellent, highly specific binding characteristics in the fermentation broth (K(d) = 1.94 micromolar; Q(max) = 222.8 mg/g), which obeyed the Langmuir isotherm and had rapid binding kinetics (equilibrium in
Language: | English |
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Year: | 2012 |
Pages: | 909-918 |
ISSN: | 18606768 and 18607314 |
Types: | Journal article |
DOI: | 10.1002/biot.201100376 |
ORCIDs: | Villadsen, John and Hobley, Timothy John |