Journal article · Ahead of Print article
Comammox Nitrospira are abundant ammonia oxidizers in diverse groundwater‐fed rapid sand filter communities : ComammoxNitrospirain drinking water biofilters
Technical University of Denmark1
Department of Environmental Engineering, Technical University of Denmark2
Water Technologies, Department of Environmental Engineering, Technical University of Denmark3
Department of Micro- and Nanotechnology, Technical University of Denmark4
Surface Engineering, Department of Micro- and Nanotechnology, Technical University of Denmark5
Department of Applied Mathematics and Computer Science, Technical University of Denmark6
The recent discovery of completely nitrifying Nitrospira demands a re‐examination of nitrifying environments to evaluate their contribution to nitrogen cycling. To approach this challenge, tools are needed to detect and quantify comammox Nitrospira. We present primers for the simultaneous quantification and diversity assessement of both comammox Nitrospira clades.
The primers cover a wide range of comammox diversity, spanning all available high quality sequences. We applied these primers to 12 groundwater‐fed rapid sand filters, and found comammox Nitrospira to be abundant in all filters. Clade B comammox comprise the majority (∼75%) of comammox abundance in all filters.
Nitrosomonadaceae were present in all filters, although at low abundance (mean = 1.8%). Ordination suggests that temperature impacts the structure of nitrifying communities, and in particular that increasing temperature favours Nitrospira. The nitrogen content of the filter material, sulfate concentration and surface ammonium loading rates shape the structure of the comammox guild in the filters.
This work provides an assay for simultaneous detection and diversity assessment of clades A and B comammox Nitrospira, expands our current knowledge of comammox Nitrospira diversity and demonstrates a key role for comammox Nitrospira in nitrification in groundwater‐fed biofilters.
Language: | English |
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Publisher: | Wiley |
Year: | 2018 |
Pages: | 1002-1015 |
ISSN: | 14622920 and 14622912 |
Types: | Journal article and Ahead of Print article |
DOI: | 10.1111/1462-2920.14033 |
ORCIDs: | 0000-0003-3178-0743 , Palomo, Alejandro , Dechesne, Arnaud and Smets, Barth F. |