Journal article
Green seaweeds (Ulva fasciata sp.) as nitrogen source for fungal cellulase production
Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Enzyme Technology, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark2
Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Department of Biotechnology and Biomedicine, Technical University of Denmark4
Kwame Nkrumah University of Science and Technology5
In developing countries, local enzyme production can help decrease the dependency of imported enzymes for bioconversion of e.g. cellulosic feedstocks, but the use of conventional nitrogen sources contributes significantly to such enzyme production cost. Use of local resources is therefore important to consider.
Green seaweeds are marine macroalgae that are rich in nitrogen, but not exploited for their nitrogen content. Cellulase production was accomplished by using cocoa pod husk (CPH) and green seaweed (GS) (Ulva fasciata sp.) as growth substrates for Polyporus ciliatus CBS 366.74 in submerged cultivation.
The nitrogen concentration of GS was comparable to that of CPH with 0.6% w/v peptone at 4% w/v substrate concentration. A decline of cellulase activity in peptone supplemented GS growth media indicated nitrogen sufficiency of GS to serve as a potential nitrogen source for the fungal growth and cellulase production.
Comparison of enzyme production on CPH growth media supplemented with either GS or peptone based on equivalent carbon to nitrogen ratios was done for two Polyporus strains namely; P. ciliatus CBS 366.74 and P. brumalis CBS 470.77. Peptone could be substituted by up to 0.6% w/v with GS at inclusion levels of 50–100% of substrate concentration to attain satisfactory cellulase productivity.
However, the cellulase productivity response varied among the two Polyporus species. This study demonstrated that green seaweeds may be used as alternative nitrogen sources for fungal cellulase production.
Language: | English |
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Publisher: | Springer Netherlands |
Year: | 2019 |
Pages: | 82 |
ISSN: | 15730972 and 09593993 |
Types: | Journal article |
DOI: | 10.1007/s11274-019-2658-1 |
ORCIDs: | Meyer, Anne S. and Thygesen, Anders |
Applied Microbiology Biochemistry, general Biomedical and Life Sciences Biotechnology Cacao Carbon Cellulase Cellulose Culture Media Environmental Engineering/Biotechnology Enzyme Assays Fermentation Ghana Glycoside Hydrolases Life Sciences Microbiology Nitrogen Polyporus Seaweed Ulva beta-Glucosidase